Mode-Locked Technology Sp. z o.o., ul. Klecińska 123, 54-413 Wroclaw,Poland info@mode-locked.com

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ModeSHG

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ModeSHG is a compact, turn-key operated Er-doped fiber laser with second harmonic generation (760-780 nm), and integrated pulse-picker for repetition rate tuning. Ideal for multiphoton microscopy and fluorescence microscopy.

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  • Pulse repetition rate: tunable from 1 to 25 MHz (integrated pulse picker)
  • Two laser outputs: main 780 nm, auxiliary 1560 nm
  • Ultrashort pulse duration (<70 fs)
  • Self-starting, maintenance-free, button-operated
  • No saturable absorber
  • Single-box solution
Central wavelengths Main output: tunable from 762 to 788 nm, FWHM > 8 nm
Pulse repetition rate (frep) Tunable from 1 to 25 MHz (frep = 25/M, where M=1, 2, …, 25)
Output pulse energy (Ep) > 1 nJ for central wavelengths 760 – 775 nm (max. 1.5 nJ)
> 0.6 nJ for central wavelengths > 777 nm
Pulse duration <70 fs for central wavelengths 760 – 775 nm
<100 fs for central wavelengths > 777 nm
Polarization Linear
Additional inputs/outputs Auxiliary output: 1560 nm +/- 10 nm, FWHM > 10 nm, FC/APC, PM
Monitor of the laser repetition frequency (frep) – BNC, >500 mV amplitude, 50Ω
Output power stability < 0.8% rms over 20 hours
Dimensions 430x330x157 mm (WxLxH), single-box solution

  • Output spectrum recorded at 25 MHz repetition rate at wavelength setting λc = 766 nm

  • ROG temporal intensity at 25 MHz repetition rate
    and λc = 766 nm

  • Output power stability over ~20 hours

  • Output pulse energy vs. repetition rate

Application notes and scientific publications

[1] Raphael Francis Aguas, Michal Dabrowski, Boyan Pan, Marcin Marzejon, Agata Kotulska, Maciej D Wojtkowski, Andrew Browne, „Refining a Human Two-Photon Imaging System,” Invest. Ophthalmol. Vis. Sci. 2024;65(7):3424.

[2] Jakub Bogusławski, Sławomir Tomczewski, Michał Dąbrowski, Katarzyna Komar, Jadwiga Milkiewicz, Grażyna Palczewska, Krzysztof Palczewski, Maciej Wojtkowski, „In vivo imaging of the human retina using a two-photon excited fluorescence ophthalmoscope,” STAR Protocols 102225, Volume 4, Issue 2 (2023)

[3] Jakub Boguslawski, Grazyna Palczewska, Slawomir Tomczewski, Jadwiga Milkiewicz, Piotr Kasprzycki, Dorota Stachowiak, Katarzyna Komar, Marcin J. Marzejon, Bartosz L. Sikorski, Arkadiusz Hudzikowski, Aleksander Głuszek, Zbigniew Łaszczych, Karol Karnowski, Grzegorz Soboń, Krzysztof Palczewski, Maciej Wojtkowski, „In vivo imaging of the human eye using a 2-photon-excited fluorescence scanning laser ophthalmoscope,” J Clin Invest. 2022;132(2):e154218.

[4] Grazyna Palczewska, Maciej Wojtkowski, Krzysztof Palczewski, „From mouse to human: Accessing the biochemistry of vision in vivo by two-photon excitation,” Progress in Retinal and Eye Research 101170, Volume 93 (2023)

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